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The 2 protein bands from 40 kDa to thirty kDa have been absent following 24 h incubation at forty five °C. For the rest of the bands, different concentrations of all three proteases retained protein bands akin to their original molar masses (Determine 6a,b). The presence of bands at low molecular pounds in fish roe before hydrolysis was unforeseen. While in the report by Ghelichi et al. [8], small peaks of a comparatively lower molecular fat array (3–fourteen kDa) ended up observed in defatted carp roe homogenate, most likely resulting from an autolytic approach happening in fish roe and/or the defatting approach resulting in the development of very low-molecular-pounds peptides.
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